FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{HA-Rich}
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General Information
Symbol
PBac{HA-Rich}
FlyBase ID
FBtp0069306
Feature type
transgenic_transposable_element
Component Allele(s)
RichTag:HA
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele RichTag:HA
Transgenic product class
genomic_DNA
(Tong et al., 2011)
wild_type
(Tong et al., 2011)
Component
Use(s)
Regulatory region(s)
Rich
Encoded product / tool
Rich
Tagged with
Tag:HA
Description

A 7.5 kb genomic fragment encompassing Rich obtained from BACR48E10, and containing a triple Tag:HA tag inserted at the N-terminus.

(Tong et al., 2011)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval optic lobe | ubiquitous
(Tong et al., 2011)
lamina anlage | intense
(Tong et al., 2011)
medulla anlage | intense
(Tong et al., 2011)
pupal stage
eye disc
(Tong et al., 2011)
pupal stage P5(ii)
lamina anlage | intense
(Tong et al., 2011)
medulla anlage | intense
(Tong et al., 2011)
pupal stage P6
salivary gland
(Tong et al., 2011)
pharate adult stage P8
lamina | intense
(Tong et al., 2011)
medulla | intense
(Tong et al., 2011)
eye photoreceptor cell | intense
(Tong et al., 2011)

Comment: in S2 cells

(Tong et al., 2011)
Additional Information
Statement
Reference

RichTag:HA protein is strongly expressed in the medulla and lamina neuropil of the optic lobe in larva and pupa.It is also expressed in the photoreceptor cells of the eye disc in pupal and pharate individuals. When expressed in S2 cells it localises to the Golgi apparatus.

(Tong et al., 2011)
 
Marker for
Reflects expression of
Rich
(Tong et al., 2011)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
605384
w*; PBac{HA-Rich}VK00037; D1/TM6B, Tb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
HA-Rich-gRE
(Tong et al., 2011)
PBac{HA-Rich}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)