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General Information
Symbol
P{10XUAS-IVS-Syn21-GFP-p10}
FlyBase ID
FBtp0073107
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
10XUAS-IVS-Syn21-GFP-p10, pJFRC81, JFRC81-10XUAS-IVS-Syn21-GFP-p10
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Encoded product / tool
Description

Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of EGFP which has been optimised for Drosophila codon usage. A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and EGFP sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the EGFP sequences.

UASt regulatory sequences (comprises 10xUAS sites plus Hsp70 sequence that includes the promoter and 203bp of 5'UTR sequence) drive expression of EGFP.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
reporter
gene
Component Alleles
Molecular data

UASt regulatory sequences (comprises 10xUAS sites plus Hsp70 sequence that includes the promoter and 203bp of 5'UTR sequence) drive expression of EGFP.

Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of EGFP which has been optimised for Drosophila codon usage. A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and EGFP sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the EGFP sequences.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Symbol Synonym
10XUAS-IVS-Syn21-GFP-p10
10xUAS-IVS-syn21-GFP-p10
JFRC81-10XUAS-IVS-Syn21-GFP-p10
P{10XUAS-IVS-Syn21-GFP-p10}
P{10xUAS-IVS-Syn21-GFP-p10}
Secondary FlyBase IDs
    References (8)