FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-HSV-TimeSTAMPa-HA-CaMKII}
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General Information
Symbol
P{UAS-HSV-TimeSTAMPa-HA-CaMKII}
FlyBase ID
FBtp0083405
Feature type
transgenic_transposable_element
Component Allele(s)
CaMKIIUAS.TimeSTAMPa
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele CaMKIIUAS.TimeSTAMPa
Transgenic product class
wild_type
(Lin et al., 2008)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
CaMKII
Tagged with
Tag:HA
TimeSTAMPa
Description

UAS regulatory sequences drive expression of a coding sequence that consists of an HSV epitope tag, a TimeSTAMPa cassette, a Tag:HA tag and CaMKII. The CaMKII protein is constitutively tagged with Tag:HA, but the presence of the TimeSTAMPa cassette means that tagging of CaMKII with the HSV epitope tag is drug-dependent, requiring the presence of a specific inhibitor (BILN-2061). In the absence of BILN-2061, the TimeSTAMPa cassette catalyzes its own cleavage from the protein sequence, and thus the upstream HSV epitope tag is removed from the CaMKII protein. In the presence of BILN-2061 this cleavage is inhibited, and thus any CaMKII protein newly synthesized in the presence of the inhibitor is tagged with the HSV epitope tag in addition to Tag:HA.

(Lin et al., 2008)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
HSV-TSa-HA-dCaMKII
(Lin et al., 2008)
HSV-TimeSTAMPa-HA-dCAMKII
(Lin et al., 2008)
P{UAS-HSV-TimeSTAMPa-HA-CaMKII}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)