Transgenic construct used to study interactions between insulators and promoters. Consists of 10 UAS sites, an FRT cassette containing the Abd-B Fab-7 insulator in the reverse orientation, the y coding region, a loxP cassette containing the promoter targeting sequences (Abd-B-PTS) and the Fab-8 insulator (nucleotides 63683 to 64582 in clone DS07696) in the reverse orientation and the mini-w gene.