Transgenic construct used to study a Polycomb Group response element (PRE) from the en locus. Generated using the pP{CaSpeR} vector; a mutated 'PRE2' en fragment (extending from -576 to -395bp relative to the en transcription start site) has been inserted upstream of the w+mC minigene. The PRE2 fragment has been altered to contain mutations that affect both z protein-binding site 'Z1' and also site A (bound by an unknown protein), with the sequence being changed from AGAGGGAGTGAACAGTGC to AGAGGGtacctACAGTGC.