FB2020_06, released Dec 22, 2020

Recombinant Construct: P{nullo-4xintron-kuk-lacZ}

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General Information
Symbol
P{nullo-4xintron-kuk-lacZ}
FlyBase ID
FBtp0093641
Feature type
transgenic_transposon
Component Allele(s)
Ecol\lacZnullo.4xintron.kuk
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Guilgur et al., 2014)
Construct components
Component allele Ecol\lacZnullo.4xintron.kuk
Description

The nullo promoter is fused upstream of 4 repeats which each consist of a fragment of the 5'UTR and intron of the kuk small transcript separated by 201bp of in-frame Ecol\lacZ sequence. The stop codon of the Ecol\lacZ sequence is followed in each case by the 3'UTR of the kuk small transcript and 300bp of genomic 3' sequence (to promote transcriptional termination).

(Guilgur et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
reporter
gene
(Guilgur et al., 2014)
Component Alleles
Allele
Ecol\lacZnullo.4xintron.kuk
Reference(s)
(Guilgur et al., 2014)
Molecular data

The nullo promoter is fused upstream of 4 repeats which each consist of a fragment of the 5'UTR and intron of the kuk small transcript separated by 201bp of in-frame Ecol\lacZ sequence. The stop codon of the Ecol\lacZ sequence is followed in each case by the 3'UTR of the kuk small transcript and 300bp of genomic 3' sequence (to promote transcriptional termination).

(Guilgur et al., 2014)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{nullo-4xintron-kuk-lacZ}
(FlyBase, 1992-)
nullo 4x intron kuk-LacZ
(Guilgur et al., 2014)
Secondary FlyBase IDs
    References (2)