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General Information
Symbol
P{13xLexAop2(KDRT.stop)myr::smGdP-V5}
FlyBase ID
FBtp0108747
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Product class / Tool use(s)
Description

Thirteen copies of the lexAop regulatory sequence are separated from a non-fluorescent 'spaghetti monster GFP' ('smGdP') marker by a KDRT cassette containing stop sequences, which prevents expression of the marker in the absence of KDR recombinase. The smGdP marker consists of 10 copies of the Tag:V5 tag inserted in groups into the backbone of the superfolder green fluorescent protein, which has been rendered non-fluorescent by mutation. A N-terminal myristoylation signal (Tag:Myr(Src64B)) is also present and the marker has been optimised for Drosophila codon usage.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Component Alleles
Reference(s)
Molecular data

Thirteen copies of the lexAop regulatory sequence are separated from a non-fluorescent 'spaghetti monster GFP' ('smGdP') marker by a KDRT cassette containing stop sequences, which prevents expression of the marker in the absence of KDR recombinase. The smGdP marker consists of 10 copies of the Tag:V5 tag inserted in groups into the backbone of the superfolder green fluorescent protein, which has been rendered non-fluorescent by mutation. A N-terminal myristoylation signal (Tag:Myr(Src64B)) is also present and the marker has been optimised for Drosophila codon usage.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{13xLexAop2(KDRT.stop)myr::smGdP-V5}
pJFRC217-13xLexAop2-KDRT>-dSTOP-KDRT>-myr::smGFP-V5
Secondary FlyBase IDs
    References (2)