There is a single nucleotide polymorphism in the cloned tRNA:CR31143 genomic DNA that is distinct from the genome reference (marked by parentheses): ATTCG TAA(G)A TGTCA GGATGGC. The variant in the reference genome is ATTCG TAA(C)A TGTCA GGATGGC. This variant does not affect the tRNA expression since it is outside of the tRNA gene, -3-nt up- stream of the normal mature tRNA gene.