A mCherry tag is introduced at the ATG of the mud open reading frame in the CH322-147E14 BAC genomic clone. This BAC clone contains ~19.5 kb of X chromosome genomic region including ~3.3 kb upstream of the mud mRNA 5' and 5.4 kb downstream of the mud 3' mRNA and thus most of the coding regions of genes proximal and distal to mud locus.