FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{GMR-Pink1.S346A.Tom20.GFP}
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General Information
Symbol
M{GMR-Pink1.S346A.Tom20.GFP}
FlyBase ID
FBtp0116638
Feature type
transgenic_transposable_element
Component Allele(s)
Pink1S346A.GMR.Tag:Mito(Tom20),GFP
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Pink1S346A.GMR.Tag:Mito(Tom20),GFP
Transgenic product class
inframe_deletion
(Zhuang et al., 2016)
missense_variant
(Zhuang et al., 2016)
polypeptide_post_translational_processing_variant
(Zhuang et al., 2016)
Component
Use(s)
Regulatory region(s)
GMR
Encoded product / tool
Pink1
Tagged with
GFP
Tag:Mito(Tom20)
Description

GMR regulatory sequences drive expression of Pink1 carrying the non-phosphorylatable mutation S346A (mutated residue is a conserved autophosphorylation site which appears to be the sole autophosphorylation site in Pink1). In addition, the N-terminal 93 amino acids of Pink1 have been replaced with the Tag:Mito(Tom20) outer mitochondrial membrane anchor signal and the protein is tagged with GFP.

(Zhuang et al., 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
83369
w*; M{GMR-Pink1.S346A.Tom20.GFP}ZH-86Fb
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
GMR-tom20-pink1S346A-GFP
(Zhuang et al., 2016)
M{GMR-Pink1.S346A.Tom20.GFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)