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General Information
Symbol
P{R35D04-p65.AD}
FlyBase ID
FBtp0117187
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
Construct components
Component allele Hsap\RELAAD.R35D04
Product class / Tool use(s)
Description

The R35D04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
molecular
tag
Component Alleles
Molecular data

The R35D04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
lobula columnar neuron LC10a

Comment: when combined with P{R22D06-GAL4.DBD} (combination referred to as 'OL0019B')

when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS2')

when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R80E07-GAL4.DBD} (combination referred to as 'OL0020B')

when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

when combined with P{R91B01-GAL4.DBD} (combination referred to as 'OL0022B')

when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

lobula columnar neuron LC10b | subset

Comment: when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS2')

when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

lobula columnar neuron LC10a | subset

Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

lobula columnar neuron LC10b

Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

when combined with P{VT040747-GAL4.DBD} (combination referred to as 'SS02663')

when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS02664')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

lobula columnar neuron LC10c

Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

when combined with P{VT040747-GAL4.DBD} (combination referred to as 'SS02663')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

lobula columnar neuron LC12

Comment: when combined with P{R55F01-GAL4.DBD} (combination referred to as 'OL0008B')

when combined with P{R65B05-GAL4.DBD} (combination referred to as 'OL0007B')

adult ventral nerve cord | restricted

Comment: when combined with P{R55F01-GAL4.DBD} (combination referred to as 'OL0008B')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

lobula columnar neuron LC10d

Comment: when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R80E07-GAL4.DBD} (combination referred to as 'OL0020B')

when combined with P{R91B01-GAL4.DBD} (combination referred to as 'OL0022B')

when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

lobula columnar neuron LC10c | subset

Comment: when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

Additional Information
Statement
Reference

Hsap\RELAAD.R35D04 labels approximately 100 cells per hemisphere - mostly LC10a with a small number of LC10b neurons - when in combination with Scer\GAL4DBD.VT043656.

In a split-GAL4 system, Hsap\RELAAD.R35D04 drives expression in lobula columnar neuron LC10a when combined with Scer\GAL4DBD.R22D06, lobula columnar neuron LC10b when combined with Scer\GAL4DBD.VT043656, lobula columnar neuron LC10b, LC10c and a single LC10a neuron when combined with Scer\GAL4DBD.R22A07, lobula columnar neuron LC10a and LC10d when combined with Scer\GAL4DBD.R80E07 or Scer\GAL4DBD.R91B01, lobula columnar neuron LC10b and LC10c when combined with Scer\GAL4DBD.VT040747, lobula columnar neuron LC10a, LC10b and LC10c when combined with Scer\GAL4DBD.R82D11, lobula columnar neuron LC10a, LC10b and LC10c and a subset of neurons in the thoracico-abdominal ganglion when combined with Scer\GAL4DBD.VT058944, lobula columnar neuron LC10a, LC10b, LC10c and LC10d when combined with Scer\GAL4DBD.R71E06, lobula columnar neuron LC10a, LC10d and a single neuron each of LC10b and LC10C when combined with Scer\GAL4DBD.VT045997, lobula columnar neuron LC12 when combined with Scer\GAL4DBD.R65B05 or LC12 and a subset of neurons in the thoracico-abdominal ganglion when combined with Scer\GAL4DBD.R55F01.

 
Marker for
Reflects expression of
Reporter construct used in assay
Images
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (12)
External Crossreferences and Linkouts ( 22 )
Linkouts
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{R35D04-p65.AD}
R35D04-p65.AD
R35D04.p65ADZp
R35D04_p65ADZp
Secondary FlyBase IDs
    References (8)