FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-AblFRET}
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General Information
Symbol
P{UAS-AblFRET}
FlyBase ID
FBtp0117628
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPUAS.AblFRET
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPUAS.AblFRET
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Avic\GFP
Description

UAS regulatory sequences drive expression of a biosensor (GB:AF440203) that reports activity of the Abl protein in vivo. The sensor consists of a phosphorylation substrate region (in this case from the mammalian CrkII protein) sandwiched between ECFP and EYFP. Phosphorylation of the substrate region results in a conformational change that induces a change in the fluorescence resonance energy transfer (FRET), while dephosphorylation reverses the FRET change (PMID:11752449).

(Kannan et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
98087
y1 w67c23; P{UAS-AblFRET}3
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Abl FRET biosensor
(Kannan et al., 2017)
P{UAS-AblFRET}
(FlyBase, 1992-)
UAS-Abl-FRET
(Marquilly et al., 2021)
Secondary FlyBase IDs
    References (5)