FB2026_02 , released June 18, 2026
Recombinant Construct: P{20xUAS-Epac1-camps}
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General Information
Symbol
P{20xUAS-Epac1-camps}
FlyBase ID
FBtp0125812
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPEpac1-camps.20xUAS
Also Known As
UAS-Epac1-camps
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPEpac1-camps.20xUAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Avic\GFP
Description

Twenty copies of the UAS regulatory sequence drive expression of two different fluorophores (CFP and YFP) which are separated by sequences encoding only the cAMP-binding domain of the Homo sapiens RAPGEF3 gene. When not bound to cAMP, Avic\GFPEpac1-camps.20xUAS supports fluorescence resonance energy transfer (FRET) from CFP to YFP. The binding of cAMP forces the CFP and YFP domains to move apart leading to a reduction in FRET levels when cAMP levels rise.

(Maiellaro et al., 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
20xUAS-Epac1-camps
(Maiellaro et al., 2016)
P{20xUAS-Epac1-camps}
(FlyBase, 1992-)
UAS-Epac1-camps
(Sun et al., 2024, Scholz et al., 2017)
Secondary FlyBase IDs
    References (5)