UASt regulatory sequences drive expression of a Hsap\TBX2 cDNA in which the coding sequence has been mutated to carry the R305H amino acid replacement (a potentially deleterious mutation identified as a candidate for the phenotype in an individual affected by a multisystem malformation disorder). The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein.