FB2026_03 , released September 17, 2026
Recombinant Construct: P{twi-lacZ.1xPE}
Open Close
General Information
Symbol
P{twi-lacZ.1xPE}
FlyBase ID
FBtp0132372
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZtwi.1xPE
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZtwi.1xPE
Transgenic product class
Component
Use(s)
Regulatory region(s)
twi_440bp_promoter
Encoded product / tool
lacZ
Description

Deletion of HindIII-EcoRI fragment, sequences from -2.9kb to -440bp.

(Jiang et al., 1991)

A single copy of the twi proximal element (PE) drives expression of lacZ.

(Jiang et al., 1992)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5
organism | ventral
(Jiang and Levine, 1993)
Additional Information
Statement
Reference

Weak expression of EcollacZ spanning the12-14 ventral-most cells of the embryo.

(Jiang and Levine, 1993)

In embryos at cellular blastoderm, expressed at low to moderate levels in the ventralmost 12-14 cells, with fuzzy boundaries at lateral borders.

(Jiang and Levine, 1993)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

construct_comment: One of a series of constructs characterizing 5\' regulatory regions of twi, in particular the dl binding sites. Additional related constructs listed under P{twi-lacZ.PEe}.

(Jiang and Levine, 1993)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{440twi-lacZ}
(FlyBase, 1996-)
P{twi-lacZ.1xPE}
(FlyBase, 1996-)
P{twi-lacZ.PE}
(FlyBase, 1996-)
Secondary FlyBase IDs
  • FBtp0005713
  • FBms0003789
  • FBtp0011925
References (7)