FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{10XUAS-ArchT-P2A-mRuby3.CAAX}
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General Information
Symbol
P{10XUAS-ArchT-P2A-mRuby3.CAAX}
FlyBase ID
FBtp0133484
Feature type
transgenic_transposable_element
Component Allele(s)
Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)
HspTP009\ArchT10xUAS.Tag:HA
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele HspTP009\ArchT10xUAS.Tag:HA
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
HspTP009\ArchT
Tagged with
Tag:HA
Description

10xUAS sequences drive expression of two coding regions that are separated by a P2A linker (this results in separate translation of each protein). The first coding sequence consists of HspTP009\ArchT (a light-gated outward proton pump) that is tagged at the C-terminal end with a Tag:HA epitope, a trafficking sequence, and an ER export sequence (represented in FlyBase by HspTP009\ArchT10xUAS.Tag:HA). The second coding sequence consists of mRuby3 that is tagged at the C-terminal end with a Tag:FLAG epitope and a Tag:CAAX(Unk) membrane targeting signal (represented in FlyBase by Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)).

(Wu et al., 2019)
Component allele Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mRuby3
Tagged with
Tag:CAAX(Unk)
Tag:FLAG
Description

10xUAS sequences drive expression of two coding regions that are separated by a P2A linker (this results in separate translation of each protein). The first coding sequence consists of HspTP009\ArchT (a light-gated outward proton pump) that is tagged at the C-terminal end with a Tag:HA epitope, a trafficking sequence, and an ER export sequence (represented in FlyBase by HspTP009\ArchT10xUAS.Tag:HA). The second coding sequence consists of mRuby3 that is tagged at the C-terminal end with a Tag:FLAG epitope and a Tag:CAAX(Unk) membrane targeting signal (represented in FlyBase by Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)).

(Wu et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
82177
w*; P{10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{10XUAS-ArchT-P2A-mRuby3.CAAX}
(FlyBase, 1992-)
UAS-ArchT-P2A-mRuby3CAAX
(Wu et al., 2019)
Secondary FlyBase IDs
    References (4)