FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-Dam-Trf2}
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General Information
Symbol
P{UAS-Dam-Trf2}
FlyBase ID
FBtp0135574
Feature type
transgenic_transposable_element
Component Allele(s)
Trf2UAS.Tag:Dam,Tag:MYC
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Trf2UAS.Tag:Dam,Tag:MYC
Transgenic product class
cDNA
(Neves and Eisenman, 2019)
wild_type
(Neves and Eisenman, 2019)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Trf2
Tagged with
Tag:Dam
Tag:MYC
Description

The P{UAS-Dam-Trf2} construct is used in the "Targeted DamID" ("TaDa") technique. UASt sequences drive expression of two open reading frames separated by stop codons (ORF1 is simply present to reduce translation of the Tag:Dam-containing ORF2, see FBrf0222171). In P{UAS-Dam-Trf2}, ORF2 consists of the Trf2 coding region (derived from the LD27895 cDNA clone) tagged with Tag:Dam and Tag:MYC.

(Neves and Eisenman, 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
80567
w*; P{UAS-Dam-Trf2}attP2
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Dam-Trf2}
(FlyBase, 1992-)
UAS-Dam-TRF2S
(Neves and Eisenman, 2019)
Secondary FlyBase IDs
    References (3)