FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-MAPPER.mCh}
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General Information
Symbol
P{UAS-MAPPER.mCh}
FlyBase ID
FBtp0140209
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPMAPPER.UAS.mCh
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPMAPPER.UAS.mCh
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Avic\GFP
Tagged with
mCherry
Description

UAS regulatory sequences drive expression of the 'MAPPER' (membrane-attached peripheral ER) marker. This marker selectively labels ER (endoplasmic reticulum) - PM (plasma membrane) junctions. It is a fusion protein composed of the signal peptide of the human STIM1 gene (HGNC:11386), GFP, the STIM1 transmembrane domain, an FKBP12-rapamycin binding (FRB) domain that is flanked on both sites by flexible linkers, and the polybasic (PB) motif of the human RIT1 gene (HGNC:10023). The GFP portion is located in the ER lumen, while the Rit PB motif binds to phosphoinositides in the plasma membrane, resulting in a constitutive localization to ER-PM junctions (details from PMID:24183667). The reporter is tagged with mCherry.

(Ugrankar et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-MAPPER.mCh}
(FlyBase, 1992-)
UAS-dMAPPER-mCherry
(Ugrankar et al., 2019)
Secondary FlyBase IDs
    References (3)