FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{Ubi-msl-2.R543A.Δ13d.FLAG}
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General Information
Symbol
M{Ubi-msl-2.R543A.Δ13d.FLAG}
FlyBase ID
FBtp0140275
Feature type
transgenic_transposable_element
Component Allele(s)
msl-2R543A.Δ13d.Ubi.Tag:FLAG
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele msl-2R543A.Δ13d.Ubi.Tag:FLAG
Transgenic product class
inframe_deletion
(Tikhonova et al., 2019)
missense_variant
(Tikhonova et al., 2019)
Component
Use(s)
Encoded product / tool
msl-2
Tagged with
Tag:FLAG
Description

A strong ubiquitin promoter drives expression of a msl-2 cDNA that has been mutated so that the protein carries a R543A amino acid substitution (this point mutation has previously been shown to be essential for DNA binding) and sequence encoding amino acid residues 642-654 has been deleted (this removes the distal part of the Clamp protein-binding domain). The coding sequence is tagged at the C-terminal end with 3xTag:FLAG.

(Tikhonova et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
MSL2R*Δ13d
(Tikhonova et al., 2019)
MSL2R*Δ13d-3xFLAG
(Tikhonova et al., 2019)
M{Ubi-msl-2.R543A.Δ13d.FLAG}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)