FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-Venus-rCamk2a.T305D.T306D-CFP}
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General Information
Symbol
P{UAS-Venus-rCamk2a.T305D.T306D-CFP}
FlyBase ID
FBtp0141657
Feature type
transgenic_transposable_element
Component Allele(s)
Rnor\Camk2aT305D.T306D.UAS.Venus,CFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Rnor\Camk2aT305D.T306D.UAS.Venus,CFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rnor\Camk2a
Description

UASt regulatory sequences drive expression of the entire Rnor\Camk2a coding sequence, mutated to carry T305D and T306D amino acid substitutions (abolishing calmodulin binding), and tagged at the N-terminal end with Venus and at the C-terminal end with a cyan fluorescent protein that has enhanced brightness (carries the S175G mutation). No change in fluorescence resonance energy transfer (FRET) between the two fluorophores is seen in response to Ca[2+] and calmodulin (details from PMID:15788767).

(Shakiryanova et al., 2011)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Venus-rCamk2a.T305D.T306D-CFP}
(FlyBase, 1992-)
T305/306D
(Shakiryanova et al., 2011)
Secondary FlyBase IDs
    References (3)