FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-nls-SCAT3}
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General Information
Symbol
P{UAS-nls-SCAT3}
FlyBase ID
FBtp0141917
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPSCAT3.UAS.Tag:NLS(Unk)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPSCAT3.UAS.Tag:NLS(Unk)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Avic\GFP
Tagged with
Tag:NLS(Unk)
Description

UAS regulatory sequences drive expression of a nuclear-localized form of "SCAT3", a genetically encoded reporter for caspase activation. SCAT3 comprises the ECFP and Venus proteins linked by an 18 amino acid peptide that includes DEVD, the caspase-3 cleavage sequence. Caspase activation can be monitored by changes in the fluorescence energy transfer (FRET) from ECFP to Venus; cleavage of the linker peptide by activated caspase abolishes the FRET (details from PMID:12527749).

(Nakajima et al., 2011)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UAS-nls-SCAT3}
(FlyBase, 1992-)
UAS-nls-SCAT3
(Nakajima et al., 2011)
UAS-nls::SCAT3
(Hoshika et al., 2020)
Secondary FlyBase IDs
    References (4)