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General Information
Symbol
P{lexAop-SCAT3}
FlyBase ID
FBtp0141918
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Avic\GFPSCAT3.lexAop
Product class / Tool use(s)
Description

lexAop regulatory sequences drive expression of "SCAT3", a genetically encoded reporter for caspase activation. SCAT3 comprises the ECFP and Venus proteins linked by an 18 amino acid peptide that includes DEVD, the caspase-3 cleavage sequence. Caspase activation can be monitored by changes in the fluorescence energy transfer (FRET) from ECFP to Venus; cleavage of the linker peptide by activated caspase abolishes the FRET (details from PMID:12527749).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Reference(s)
Molecular data

lexAop regulatory sequences drive expression of "SCAT3", a genetically encoded reporter for caspase activation. SCAT3 comprises the ECFP and Venus proteins linked by an 18 amino acid peptide that includes DEVD, the caspase-3 cleavage sequence. Caspase activation can be monitored by changes in the fluorescence energy transfer (FRET) from ECFP to Venus; cleavage of the linker peptide by activated caspase abolishes the FRET (details from PMID:12527749).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lexAop-SCAT3}
lexAop-SCAT3
Secondary FlyBase IDs
    References (2)