FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UASp-α-Cat.R.H1.HA}
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General Information
Symbol
P{UASp-α-Cat.R.H1.HA}
FlyBase ID
FBtp0141966
Feature type
transgenic_transposable_element
Component Allele(s)
α-CatH1.UASp.R.Tag:HA
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele α-CatH1.UASp.R.Tag:HA
Transgenic product class
missense_variant
(Sarpal et al., 2019)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
α-Cat
Tagged with
Tag:HA
Description

UASp regulatory sequences drive expression of α-Cat that has a mutation within the α1-helix of the actin-binding domain (REAM to GSGS change starting at residue 683), tagged at the C-terminal end with Tag:HA. The α-Cat sequence has been modified to render it resistant to RNAi by either P{UAS-α-Cat.RNAi.1} (5'-ATGTTAAAA-3' changed to 5'-ATGCTGAAG-3' at the start of the coding region) or P{TRiP.HMS00317} (5'-GCAGCATCGATATTGACTGTT-3' changed to 5'-GCCGCCTCCATCCTGACCGTG-3' in the M2 domain).

(Sarpal et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UASp-α-Cat.R.H1.HA}
(FlyBase, 1992-)
UAS-α-CatR-H1
(Sarpal et al., 2019)
Secondary FlyBase IDs
    References (3)