FB2026_02 , released June 18, 2026
Recombinant Construct: P{Act(FRT.polyA)fz-mKate-sfGFP}
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General Information
Symbol
P{Act(FRT.polyA)fz-mKate-sfGFP}
FlyBase ID
FBtp0143495
Feature type
transgenic_transposable_element
Component Allele(s)
fzAct.FRT.mKate,sfGFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele fzAct.FRT.mKate,sfGFP
Transgenic product class
wild_type
(Ressurreição et al., 2018)
Component
Use(s)
Regulatory region(s)
Actin
Encoded product / tool
fz
Tagged with
mKate
sfGFP
Also carries
FRT
Description

An Actin promoter is separated from the fz coding region (tagged with sfGFP and mKate) by an FRT cassette containing polyA signals. In the presence of FLP recombinase, excision of the FRT cassette allows expression of the fz-sfGFP-mKate fusion protein, which can be used as a "fluorescent timer" to study fz turnover, due to the different maturation rates of the two fluorophores.

(Ressurreição et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
Act > STOP>fz::sfGFP-mKate
(Tan and Strutt, 2025)
Act >STOP > fz-mKate2-sfGFP
(Carayon et al., 2025)
Act>>fz-mKate2sfGFP
(Carayon et al., 2025)
Act>>fzmKate2sfGFP
(Carayon et al., 2025)
ActP-FRT-polyA-FRT-fz-mKate-sfGFP
(Ressurreição et al., 2018)
ActP-FRT-polyA-FRT-fz-mKate2-sfGFP
(Carayon et al., 2025)
P{Act(FRT.polyA)fz-mKate-sfGFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (5)