FlyBase Recombinant Construct Report: P{UAS-TetherRFP}

UAS regulatory sequences drive expression of RFP(Unk) that is tagged at the N-terminal end (via a AEAAAKEAAAKEAAAKA linker) with a Tag:Mito(hAKAP1) mitochondrial localization signal and at the C-terminal end with a 39 amino acid sequence from the Miga FFAT motif region (residues 229-267). The Miga FFAT sequence interacts with Vap33 protein (an endoplasmic reticulum protein), and thus the protein produced by this transgene can be used as a molecular tether between the endoplasmic reticulum and mitochondria.

(Xu et al., 2020)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Miga^{Tether.UAS.RFP}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{UAS-TetherRFP}

(FlyBase, 1992-)

UAS-Tether-RFP

(Xu et al., 2022)

UAS-TetherRFP

(Xu et al., 2020)

Secondary FlyBase IDs

References (4)

Report Sections

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