FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-Hph.gRNAx2.pCFD6}
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General Information
Symbol
P{UAS-Hph.gRNAx2.pCFD6}
FlyBase ID
FBtp0144648
Feature type
transgenic_transposable_element
Component Allele(s)
HphUAS.gRNAx2.pCFD6
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele HphUAS.gRNAx2.pCFD6
Transgenic product class
sgRNA
(Texada et al., 2019)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hph
Description

UAS regulatory sequences drive expression of double guide RNAs targeting the Hph genomic region encoding the dioxygenase domain common to all isoforms (target sites: GTATCGTGTTAACACGATGA, GAATATCAACTGGGATGCGC).

(Texada et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Hph.gRNAx2.pCFD6}
(FlyBase, 1992-)
UAS-HphKO
(Texada et al., 2019)
Secondary FlyBase IDs
    References (3)