FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-PMCA-kisser-pHerry}
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General Information
Symbol
P{UAS-PMCA-kisser-pHerry}
FlyBase ID
FBtp0145784
Feature type
transgenic_transposable_element
Component Allele(s)
PMCAkisser.UAS.pHerry
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele PMCAkisser.UAS.pHerry
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
PMCA
Tagged with
mCherry
pHluorinSE
Tag:HA
Tag:MYC
Tag:SS(wg)
Tag:TM(hPDGFRB)
Description

UAS regulatory sequences drive expression of a fusion protein composed of the Tag:SS(wg) signal peptide, a Tag:HA epitope, a Tag:MYC epitope, a transmembrane domain (Tag:TM(hPDGFRB)), pHluorinSE, mCherry and a modified carboxy terminus of PMCA (the Cam binding site in the PMCA sequence has been scrambled to prevent unwanted oligomerization or cleavage by calpains). The configuration of the fusion protein results in the epitope tags being extracellular, while the pHluorinSE, mCherry and PMCA portions are intracellular (the carboxy terminus of PMCA is normally intracellular in the endogenous protein).

(Stawarski et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-PMCA-kisser-pHerry}
(FlyBase, 1992-)
UAS-PMCA-kisser-pHerry
(Stawarski et al., 2018)
Secondary FlyBase IDs
    References (3)