FB2026_03 , released September 17, 2026
Recombinant Construct: P{pCFD4-loaf.sgRNA}
Open Close
General Information
Symbol
P{pCFD4-loaf.sgRNA}
FlyBase ID
FBtp0147969
Feature type
transgenic_transposable_element
Component Allele(s)
loafgRNA.snRNA:U6:96Aa.snRNA:U6:96Ac
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele loafgRNA.snRNA:U6:96Aa.snRNA:U6:96Ac
Transgenic product class
sgRNA
(Douthit et al., 2021)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Aa
snRNA:U6:96Ac
Encoded product / tool
loaf
Description

The U6:1 (snRNA:U6:96Aa) and U6:3 (snRNA:U6:96Ac) promoters (from the pCFD4 vector) regulate expression of two sgRNA sequences (GCTGGTGATTACGTCGGTGA and TGCGGGACCATCCGGGTACC) targeting loaf.

(Douthit et al., 2021)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{pCFD4-loaf.sgRNA}
(FlyBase, 1992-)
pCFD4-loaf sgRNAs
(Douthit et al., 2021)
Secondary FlyBase IDs
    References (3)