FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UASp-htl.Opto}
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General Information
Symbol
P{UASp-htl.Opto}
FlyBase ID
FBtp0148779
Feature type
transgenic_transposable_element
Component Allele(s)
htlOpto.UASp
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele htlOpto.UASp
Transgenic product class
inframe_deletion
(Yadav et al., 2021)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
htl
Tagged with
CRY2(PHR)
mCherry
Tag:Myr(Src42A)
Description

UASp regulatory sequences drive expression of the cytoplasmic domain of htl, tagged at the N-terminal end with the Tag:Myr(Src42A) membrane localization signal and at the C-terminal end with CRY2(PHR) and with mCherry. In response to blue light of 488nm, oligomerization of the protein occurs (due to the presence of the the CRY2(PHR)-mCherry portion). This allows the active htl receptor (a dimer) to be induced in a light-dependent manner, independent of ligand.

(Yadav et al., 2021)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Opto-htl
(Yadav et al., 2021)
P{UASp-htl.Opto}
(FlyBase, 1992-)
UAS-htl-Cry2mCherry
(Yadav et al., 2021)
Secondary FlyBase IDs
    References (3)