UASp regulatory sequences drive expression of egl that carries two point mutations (S965K and S969R) which disrupt the interaction with ctp protein. The coding sequence is tagged at the C-terminal end with GFP followed by a flexible linker and the leucine zipper from the Saccharomyces cerevisiae GCN4 gene (a dimerization domain). Silent mutations have been introduced that make the construct refractory to the P{TRiP.GL01170} RNAi line.