The PBac{brp(RSRT.Stop)smFPV5-2A-lexA::VP16} construct contains brp regulatory and coding sequences (genomic fragment of ~50kb) in which the stop codon has been replaced by an RSRT cassette containing transcriptional and translational stop sequences, followed by 'smFPV5' (multiple copies of the Tag:V5 tag embedded within an inactive, non-fluorescent version of GFP), the 2A peptide and a lexA::VP16 driver. Sequences downstream of the stop cassette are not translated, and thus the brp protein produced is not tagged and no lexA::VP16 protein is produced. In the presence of RSR recombinase, the RSRT cassette is excised, leaving a single RSRT site. The brp protein produced is now tagged with smFPV5, and the presence of the 2A peptide allows the lexA::VP16 driver to be cotranslated as a separate protein, under the control of the brp regulatory sequences.

The PBac{brp(RSRT.Stop)smFPV5-2A-lexA::VP16} construct contains brp regulatory and coding sequences (genomic fragment of ~50kb) in which the stop codon has been replaced by an RSRT cassette containing transcriptional and translational stop sequences, followed by 'smFPV5' (multiple copies of the Tag:V5 tag embedded within an inactive, non-fluorescent version of GFP), the 2A peptide and a lexA::VP16 driver. Sequences downstream of the stop cassette are not translated, and thus the brp protein produced is not tagged and no lexA::VP16 protein is produced. In the presence of RSR recombinase, the RSRT cassette is excised, leaving a single RSRT site. The brp protein produced is now tagged with smFPV5, and the presence of the 2A peptide allows the lexA::VP16 driver to be cotranslated as a separate protein, under the control of the brp regulatory sequences.