FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-comm.2PY-AY.monoUb.HA}
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General Information
Symbol
P{UAS-comm.2PY-AY.monoUb.HA}
FlyBase ID
FBtp0158536
Feature type
transgenic_transposable_element
Component Allele(s)
comm2PY-AY.monoUb.UAS.Tag:HA
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele comm2PY-AY.monoUb.UAS.Tag:HA
Transgenic product class
missense_variant
(Ing et al., 2007)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
comm
Tagged with
Tag:HA
Description

UASt regulatory sequences drive expression of comm containing mutations in the two PY motifs that are normally responsible for Nedd4 binding (carries the amino acid replacements P221A and P230A). The comm coding sequence is fused in frame with a single mutant ubiquitin moiety that cannot itself become polyubiquitinated (contains amino acid substitutions K11R, K29R, K48R, K63R, G75A and G76A) and is tagged at the C-terminal end with a triple Tag:HA tag.

(Ing et al., 2007)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-comm.2PY-AY.monoUb.HA}
(FlyBase, 1992-)
UAS-comm(2PY->AY-monoUb)
(Ing et al., 2007)
Secondary FlyBase IDs
    References (3)