FB2026_03 , released September 17, 2026
Recombinant Construct: M{ninaE-mch-T2A-GFP-FLAG-CL1}
Open Close
General Information
Symbol
M{ninaE-mch-T2A-GFP-FLAG-CL1}
FlyBase ID
FBtp0160132
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPUAS.CL1.Tag:FLAG.T2A-mCh
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPUAS.CL1.Tag:FLAG.T2A-mCh
Transgenic product class
Component
Use(s)
Regulatory region(s)
ninaE
Encoded product / tool
GFP
Tagged with
Tag:degron(CL1)
Tag:FLAG
Description

The ninaE promoter drives expression of a bicistronic transcript that encodes two open reading frames (ORFs) separated by a 2A linker (this results in separate translation of each protein). The first ORF encodes mCherry (internal control). The second ORF encodes a proteasome activity reporter that consists of GFP tagged with a degradation signal (Tag:degron(CL1)) plus Tag:FLAG.

(Zhao et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{ninaE-mch-T2A-GFP-FLAG-CL1}
(FlyBase, 1992-)
mCherry-T2A-GFP-FLAG-Cl1
(Zhao et al., 2023)
Secondary FlyBase IDs
    References (3)