FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{Ubi-ERK-KTR.GFP}
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General Information
Symbol
P{Ubi-ERK-KTR.GFP}
FlyBase ID
FBtp0167480
Feature type
transgenic_transposable_element
Component Allele(s)
Mmus\Elk1Ubi.KTR.GFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Mmus\Elk1Ubi.KTR.GFP
Transgenic product class
Component
Use(s)
Encoded product / tool
Mmus\Elk1
Description

A Ubiquitin promoter drives expression of a 'ERK-KTR' kinase translocation reporter of MAPK activity, a fusion protein composed of the ERK docking site from the Mmus\Elk1 gene, a nuclear localization signal (NLS), a nuclear export signal (NES) and GFP. Both the NLS and NES have been mutated to contain consensus phosphorylation sites for MAPK, such that phosphorylation decreases the activity of the NLS and increases the activity of the NES. This results in the protein shuttling from the nucleus to the cytoplasm in response to MAPK phosphorylation (details from PMID:24949979).

(Cachoux et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{Ubi-ERK-KTR.GFP}
(FlyBase, 1992-)
Ubi-KTR:GFP
(Cachoux et al., 2023)
Secondary FlyBase IDs
    References (3)