FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-EGFPdark}
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General Information
Symbol
P{UAS-EGFPdark}
FlyBase ID
FBtp0168609
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPEGFPdark.UAS
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPEGFPdark.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
EGFP
Tagged with
Tag:CS(TEVp)
Description

UASt sequences regulate expression of a fusion protein composed of EGFP, a Tag:CS(TEVp) cleavage site and a quenching peptide. The presence of the quenching peptide (a 27 residue peptide derived from the transmembrane region of influenza M2) diminishes fluorescence of EGFP by causing its tetramerization (details from PMID:21558267). In the presence of TEVp, cleavage at the Tag:CS(TEVp) site removes the quenching peptide, increasing EGFP fluorescence.

(Li et al., 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-EGFPdark}
(FlyBase, 1992-)
UAS-GFPdark
(Li et al., 2016)
Secondary FlyBase IDs
    References (3)