FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{brk(FRT.mCh-CAAX)brk.allSEwt.VH}
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General Information
Symbol
M{brk(FRT.mCh-CAAX)brk.allSEwt.VH}
FlyBase ID
FBtp0169814
Feature type
transgenic_transposable_element
Component Allele(s)
brkallSEwt.FRT(mCh-CAAX).VH
Disc\RFPmCh.brk.allSEwt.Tag:CAAX(Unk)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele brkallSEwt.FRT(mCh-CAAX).VH
Transgenic product class
wild_type
(Gafner et al., 2013)
Component
Use(s)
Regulatory region(s)
brk
Encoded product / tool
brk
Tagged with
Tag:polyHis
Tag:V5
Also carries
FRT
Description

A 32kb genomic BAC fragment that encompasses brk. A multi-tag cassette composed of 3xTag:V5 and Tag:polyHis has been inserted at the C-terminal end of the brk coding sequence. An FRT cassette that encodes the mCherry coding sequence, tagged with Tag:CAAX(Unk) and followed by stop sequences, has been inserted in the brk 5'UTR. In the absence of FLP recombinase, the Tag:CAAX(Unk)-tagged mCherry sequence is expressed under the control of endogenous brk regulatory sequences, and the brk coding sequence is not expressed.

(Gafner et al., 2013)
Component allele Disc\RFPmCh.brk.allSEwt.Tag:CAAX(Unk)
Transgenic product class
Component
Use(s)
Regulatory region(s)
brk
Encoded product / tool
mCherry
Tagged with
Tag:CAAX(Unk)
Also carries
FRT
Description

A 32kb genomic BAC fragment that encompasses brk. A multi-tag cassette composed of 3xTag:V5 and Tag:polyHis has been inserted at the C-terminal end of the brk coding sequence. An FRT cassette that encodes the mCherry coding sequence, tagged with Tag:CAAX(Unk) and followed by stop sequences, has been inserted in the brk 5'UTR. In the absence of FLP recombinase, the Tag:CAAX(Unk)-tagged mCherry sequence is expressed under the control of endogenous brk regulatory sequences, and the brk coding sequence is not expressed.

(Gafner et al., 2013)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{brk(FRT.mCh-CAAX)brk.allSEwt.VH}
(FlyBase, 1992-)
allSEwt>mCherry-CAAX>brk-V5-His
(Gafner et al., 2013)
Secondary FlyBase IDs
    References (3)