FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UASp(FRT.His3.3A-EGFP.S31A)His3.3A-mCherry.S31A}
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General Information
Symbol
P{UASp(FRT.His3.3A-EGFP.S31A)His3.3A-mCherry.S31A}
FlyBase ID
FBtp0170293
Feature type
transgenic_transposable_element
Component Allele(s)
His3.3AS31A.UASp.EGFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele His3.3AS31A.UASp.EGFP
Transgenic product class
missense_variant
(Chandrasekhara et al., 2023)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
His3.3A
Tagged with
EGFP
Also carries
FRT
Description

UASp regulatory sequences are fused upstream of a FRT cassette containing the His3.3A coding region, mutated to carry a S31A amino acid substitution and tagged with EGFP. The cassette is followed by the His3.3A coding region, mutated to carry a S31A amino acid substitution and tagged with the mCherry fluorescent protein.

(Chandrasekhara et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UASp(FRT.His3.3A-EGFP.S31A)His3.3A-mCherry.S31A}
(FlyBase, 1992-)
UASp-FRT-H3.3S31A-EGFP-PolyA-FRT-H3.3S31A-mCherry
(Chandrasekhara et al., 2023)
UASp-FRT-H3.3S31A-GFP-PolyA-FRT-H3.3S31A-mCherry
(Chandrasekhara et al., 2023)
Secondary FlyBase IDs
    References (3)