UASt sequences regulate expression of N in which a. the N-terminal EGF repeat containing part of the extracellular domain has been replaced by Tag:LBD(FSHR) and b. the juxtamembrane NRR (negative regulatory region) has been replaced with the NRR from the orthologous Cele\lin-12 gene. The coding sequence is tagged with mCherry just upstream of the juxtamembrane NRR (negative regulatory region). A single FRT site is present in between the UASt and coding sequences.