FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{mt-MRP-EGFP}
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General Information
Symbol
P{mt-MRP-EGFP}
FlyBase ID
FBtp0171018
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPEGFP.mt.MRP
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPEGFP.mt.MRP
Transgenic product class
Component
Use(s)
Encoded product / tool
EGFP
Description

A RNA polymerase III promoter drives expression of a transcription unit composed of a non-coding leader (NCL) sequence (from the RNaseMRP:RNA gene), a translation initiation sequence and the EGFP open reading frame (ORF). The ORF has been engineered for mitochondrial translation and a UGA cytosolic stop codon (that encodes the Trp residue in mitochondria) has been introduced at codon 58. The presence of the NCL sequence targets the RNA to the mitochondrion and the presence of the early cytosolic stop codon is designed to allow expression of the full-length EGFP ORF in the mitochondrion but not in the cytosol.

(Markantone et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{mt-MRP-EGFP}
(FlyBase, 1992-)
mt-TRESPro-MRP::EGFP
(Markantone et al., 2018)
Secondary FlyBase IDs
    References (3)