8xlexAop sequences regulate expression of a transcript that encodes two open reading frames separated by a T2A linker (this results in separate translation of each protein). Designed to be used as a control for the P{8XlexAop-PiGM-Iq} transgene. The first open reading frame encodes a fusion protein in which a minimal regulator of G protein signaling (RGS) domain is fused to a mutated form of CRY2(PHR) (contains a D387A mutation, which renders it light-insensitive). The minimal RGS domain consists of the Rnor\Rgs2 gene from which the first 53 amino acids have been deleted; this removes an adenylate cyclase inhibiting peptide, plus G protein-coupled receptor (GPCR) targeting and membrane association sequences, and thus the tagged RGS domain is located in the cytosol and does not bind GTP-bound Gαq. The second open reading frame encodes EGFP tagged at the N-terminal end with CIBN(ΔNLS) and at the C-terminal end with a Tag:CAAX(Unk) motif that targets the protein to the membrane.

8xlexAop sequences regulate expression of a transcript that encodes two open reading frames separated by a T2A linker (this results in separate translation of each protein). Designed to be used as a control for the P{8XlexAop-PiGM-Iq} transgene. The first open reading frame encodes a fusion protein in which a minimal regulator of G protein signaling (RGS) domain is fused to a mutated form of CRY2(PHR) (contains a D387A mutation, which renders it light-insensitive). The minimal RGS domain consists of the Rnor\Rgs2 gene from which the first 53 amino acids have been deleted; this removes an adenylate cyclase inhibiting peptide, plus G protein-coupled receptor (GPCR) targeting and membrane association sequences, and thus the tagged RGS domain is located in the cytosol and does not bind GTP-bound Gαq. The second open reading frame encodes EGFP tagged at the N-terminal end with CIBN(ΔNLS) and at the C-terminal end with a Tag:CAAX(Unk) motif that targets the protein to the membrane.