FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAS-NbALFA.6mut.mCh}
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General Information
Symbol
M{UAS-NbALFA.6mut.mCh}
FlyBase ID
FBtp0171493
Feature type
transgenic_transposable_element
Component Allele(s)
Zzzz\VHHNbALFA.6mut.UAS.mCherry
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Zzzz\VHHNbALFA.6mut.UAS.mCherry
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Zzzz\VHH
Tagged with
mCherry
Description

UAS sequences regulate expression of the NbALFA nanobody, which has been mutated to carry 6 amino acid substitutions (T25V, R65V, T75R, C100V, Q116H and S124F), and is tagged with mCherry. The encoded mutant protein appears to be destabilized in the absence of Tag:ALFA (its target peptide), but also does not accumulate at the site of Tag:ALFA-tagged proteins, most likely due to loss of target affinity.

(Aguilar et al., 2024)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{UAS-ALFANb6mut-mCh, w+}
(Aguilar et al., 2024)
M{UAS-NbALFA.6mut.mCh}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)