FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAS-FSHR-lexA::VP16.synNotch}
Open Close
General Information
Symbol
M{UAS-FSHR-lexA::VP16.synNotch}
FlyBase ID
FBtp0172142
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele Ecol\lexAFSHR.UAS.synNotch
Transgenic product class
Component
Use(s)
Description

UASt sequences regulate expression of N in which a. the N-terminal EGF repeat containing part of the extracellular domain has been replaced by sequence encoding a signal peptide and Tag:LBD(FSHR), b. the juxtamembrane NRR (negative regulatory region) has been replaced with the wild-type A2 domain (a force sensor) of the Homo sapiens VWF gene (HGNC:12726) and c. the cytosolic domain of N has been replaced with sequence encoding the lexA::VP16 driver. The coding sequence is tagged with mCherry just upstream of the A2 domain. A single FRT site is present in between the UASt and coding sequences. The protein encodes a synthetic receptor that is expressed at the cell surface. Receptor activation occurs at the interface between cells expressing the receptor and those expressing a protein that includes Tag:LIG(FSHb) (in the presence of a source of Tag:LIG(CGA))), resulting in cleavage which allows the lexA::VP16 driver to translocate to the nucleus.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{UAS-FSHR-lexA::VP16.synNotch}
UAS>FSHR-synNotch
Secondary FlyBase IDs
    References (3)