FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-hCBX7.CD.i2Dam}
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General Information
Symbol
P{UAS-hCBX7.CD.i2Dam}
FlyBase ID
FBtp0173554
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\CBX7CD.UAS.Tag:Dam
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\CBX7CD.UAS.Tag:Dam
Transgenic product class
inframe_deletion
(van den Ameele et al., 2025)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\CBX7
Tagged with
Tag:Dam
Tag:NLS(SV40-largeT)
Description

UASt regulatory sequences are fused upstream of two open reading frames separated by stop codons (the upstream ORF is simply present to reduce translation of the downstream ORF). The downstream ORF encodes the chromodomain of Hsap\CBX7 (amino acids 7-61), which recognizes and binds H3K27me3 (His3 triply methylated at position K27), tagged at the C-terminal end with 2xTag:NLS(SV40-largeT) and with Tag:Dam (an intron has been introduced into the Tag:Dam coding sequence, which greatly improves cloning efficiency by preventing toxicity in bacteria).

(van den Ameele et al., 2025)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{UAS-hCBX7.CD.i2Dam}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)