FB2026_03 , released September 17, 2026
Recombinant Construct: PBac{zk-Cas9.D10A}
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General Information
Symbol
PBac{zk-Cas9.D10A}
FlyBase ID
FBtp0175535
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9D10A.zk
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9D10A.zk
Transgenic product class
Component
Use(s)
Regulatory region(s)
Kr
zen
Encoded product / tool
Cas9(D10A)
Tagged with
Tag:NLS(Unk)
Description

The zen ventral repression element and two copies of the Kr AD1 element regulate expression of the Cas9(D10A) nickase. The nickase coding sequence is tagged with nuclear localization signals at both the N-terminal and C-terminal ends of the open reading frame.

(Shen et al., 2026)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
607571
w*; PBac{zk-Cas9.D10A}VK00020/TM6B, Tb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{zk-Cas9.D10A}
(FlyBase, 1992-)
zk-D10A
(Shen et al., 2026)
Secondary FlyBase IDs
    References (3)