FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity

ID (Ontology)

GO:0000224 (Gene Ontology)

Definition

Catalysis of the reaction: 4-N-(N-acetyl-D-glucosaminyl)-protein + H2O = N-acetyl-beta-D-glucosaminylamine + peptide L-aspartate. This reaction is the hydrolysis of an N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the N-acetyl-D-glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and the peptide containing an aspartic residue.[ EC:3 ]

Also Known As

"PNGase"

Comment

Links to External Ontologies

QuickGO data AmiGO data

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Direct annotations: records annotated with this exact term (child terms are NOT included)

Data Class	Field	Direct annotations
Genes (FBgn)	GO_MOLECULAR_FUNCTION

Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Genes
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity (all annotations which use CV term, excluding "NOT" statements)
enables \| peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity

Spanning Tree (Parents/Children)

  hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
   |__hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
       |__peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity  1 rec.

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
Part of
Synonyms & Secondary IDs
Synonyms
	"glycopeptidase activity" BROAD[ EC:3 ] "glycopeptide N-glycosidase activity" BROAD[ EC:3 ] "jack-bean glycopeptidase" NARROW[ EC:3 ] "N-glycanase activity" RELATED[ EC:3 ] "N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase activity" RELATED[ EC:3 ] "N-oligosaccharide glycopeptidase activity" RELATED[ EC:3 ] "peptide:N-glycanase" RELATED "PNGase" EXACT "PNGase A" RELATED[ EC:3 ] "PNGase F" RELATED[ EC:3 ]
Secondary IDs

External Crossreferences & Linkouts
	EC:3.5.1.52 MetaCyc:3.5.1.52-RXN Reactome:R-HSA-8850594 "Deglycosylation complex hydrolyses N-glycans from unfolded glycoproteins"