FB2026_03 , released September 17, 2026
CV Term Report
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General Information
Term peptidyl-tRNA hydrolase activity ID (Ontology) GO:0004045 (Gene Ontology)
Definition Catalysis of the reaction: an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H+.
Also Known As"aminoacyl-tRNA hydrolase reaction"
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Genes (FBgn)GO_MOLECULAR_FUNCTION
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 peptidyl-tRNA hydrolase activity (all annotations which use CV term, excluding "NOT" statements)
     enables | peptidyl-tRNA hydrolase activity       --
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hydrolase activity, acting on ester bonds
 |__carboxylic ester hydrolase activity___
catalytic activity, acting on RNA         |
 |__catalytic activity, acting on a tRNA__|
                                          peptidyl-tRNA hydrolase activity  5 rec.
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Is a carboxylic ester hydrolase activity
catalytic activity, acting on a tRNA
Part of
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Synonyms
  • "aminoacyl-transfer ribonucleate hydrolase activity" RELATED[ EC:3 ]
    "aminoacyl-tRNA aminoacylhydrolase activity" RELATED[ EC:3 ]
    "aminoacyl-tRNA hydrolase reaction" EXACT
    "D-tyrosyl-tRNA hydrolase activity" NARROW[ PubMed:15292242 ]
    "N-substituted aminoacyl transfer RNA hydrolase activity" RELATED[ EC:3 ]
Secondary IDs
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EC:3.1.1.29
MetaCyc:AMINOCYL-TRNA-HYDROLASE-RXN
Reactome:R-HSA-9937374 "MTRFR:MTRES1:39S ribosomal subunit:mRNA:peptidyl-tRNA:MALSU1:MIEF1:NDUFAB1 dissociates"
Reactome:R-HSA-9937719 "ICT1 hydrolyzes the peptidyl-tRNA bond of the nascent polypeptide"
RHEA:54448
RHEA:81979