FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

RNA polymerase II preinitiation complex assembly

ID (Ontology)

GO:0051123 (Gene Ontology)

Definition

The formation of a large multiprotein-DNA complex that self-assembles on gene promoter through the sequential recruitment of the general initiation factors that compose the preinitiation complex (PIC) (which may include TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH complexes). The PIC engages RNA polymerase II on its DNA template strand and sparks polymerization of the first few RNA nucleotides of the nascent transcript, of which 8 are base-paired with the DNA template within a DNA bubble. PIC assembly may result in a pause step, which marks the end of the PIC assembly and may be followed by promoter clearance (exact synonym: promoter escape). For RNA polymerase II PIC assembly is preceded by the formation of a nucleosome-free region that allows the transcription machinery to access the promoter DNA.[ PubMed:15020047 PubMed:31300188 ]

Also Known As

"RNA polymerase II transcription PIC biosynthesis" ; "RNA polymerase II transcription PIC formation" ; "RNA polymerase II transcriptional preinitiation complex assembly" (for all, see Synonyms field below)

Comment

Links to External Ontologies

QuickGO data AmiGO data

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Direct annotations: records annotated with this exact term (child terms are NOT included)

Data Class	Field	Direct annotations
Genes (FBgn)	GO_BIOLOGICAL_PROCESS

Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Genes
RNA polymerase II preinitiation complex assembly (all annotations which use CV term, excluding "NOT" statements)
involved_in \| RNA polymerase II preinitiation complex assembly

Spanning Tree (Parents/Children)

DNA-templated transcription initiation
 |__transcription initiation at RNA polymerase II promoter__
 |__transcription preinitiation complex assembly____________|
transcription by RNA polymerase II                          |
 |__transcription initiation at RNA polymerase II promoter__|
protein-DNA complex assembly                                |
 |__transcription preinitiation complex assembly____________|
                                                            RNA polymerase II preinitiation complex assembly  19 rec.
                                                             |__regulation of RNA polymerase II transcription preinitiation complex assembly 4 rec.
                                                                 |__negative regulation of RNA polymerase II transcription preinitiation complex assembly 1 rec.
                                                                 |__positive regulation of RNA polymerase II transcription preinitiation complex assembly 3 rec.

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	transcription preinitiation complex assembly
Part of	transcription initiation at RNA polymerase II promoter
Synonyms & Secondary IDs
Synonyms
	"RNA polymerase II transcription PIC biosynthesis" EXACT "RNA polymerase II transcription PIC formation" EXACT "RNA polymerase II transcriptional preinitiation complex assembly" EXACT "RNA polymerase II transcriptional preinitiation complex formation" EXACT
Secondary IDs

External Crossreferences & Linkouts