FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

bioluminescence resonance energy transfer

ID (Ontology)

MI:0012 (Molecular Interactions)

Definition

In this variation of the FRET assay the donor fluorophore is replaced by a luciferase (typically Renilla luciferase). In the presence of its substrate, the luciferase catalyses a bioluminescent reaction that excites the acceptor fluorophore through a resonance energy transfer mechanism. As with FRET the energy transfer occurs only if the protein fused to the luciferase and the one fused to the acceptor fluorophore are in close proximity (10-100 Angstrom).[ PubMed:9874787 ]

Also Known As

"BRET" ; "bret" ; "LRET"

Comment

Links to External Ontologies

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
bioluminescence resonance energy transfer (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  biophysical
   |__fluorescence technology
       |__bioluminescence resonance energy transfer  8 rec.

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Relationships
Is a	fluorescence technology
Part of
Synonyms & Secondary IDs
Synonyms
	"BRET" EXACT PSI-MI-alternate "bret" EXACT PSI-MI-short "LRET" EXACT PSI-MI-alternate
Secondary IDs

External Crossreferences & Linkouts