FB2026_03 , released September 17, 2026
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Term filter binding ID (Ontology) MI:0049 (Molecular Interactions)
Definition A method in which separation depends upon the ability of one participant to bind to a filter or membrane which the other participants do not. Molecules interacting with the bound molecule will also be retain on the filter. For example, proteins expressed by different clones of an expression library are bound to a nitrocellulose membrane, by colony (bacterial library) or plaque (phage library) blotting. A labelled protein can then be used as a probe to identify clones expressing proteins that interact with the probe. Interactions occur on the nitrocellulose filters. The method is highly general and therefore widely applicable. A variety of approaches can be used to label the ligand, alternatively the ligand can be detected by a specific antibody.[ PubMed:7708014 ]
Also Known As"Filter overlay assay"
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 filter binding (all annotations which use CV term, excluding "NOT" statements)
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  affinity technology
   |__solid phase assay
       |__filter binding  23 rec.
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  • "dot blot" RELATED
    "Filter overlay assay" EXACT PSI-MI-alternate
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