FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

phage display

ID (Ontology)

MI:0084 (Molecular Interactions)

Definition

Peptide sequences or entire proteins can be displayed on phage capsids by fusion to coat proteins to generate a library of fusion phages each displaying a different peptide. Such a library can then be exploited to identify specific phages that display peptides that bind to any given bait molecule for instance an antibody. The selection is performed by a series of cycles of affinity purification known as panning. The bait protein, immobilized on a solid support (plastic, agarose, sepharose, magnetic beads and others) is soaked in the phage mixture and that phage that remains attached to the bait is amplified and carried through a further affinity purification step. Each cycle results in an approximately 1,000-fold enrichment of specific phage and after a few selection rounds (2-4), DNA sequencing of the tight-binding phage reveals only a small number of sequences. Phage display panning experiments can be carried out either on libraries of peptides of random amino acid sequence or on libraries of displaying natural peptides obtained by inserting cDNA fragments into the phage vector (cDNA libraries). Libraries have been assembled on several different phages (Fd, Lambda or T7).[ PubMed:10975452 PubMed:7708014 ]

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Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
phage display (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  affinity technology
   |__display technology
       |__phage display  19 rec.
           |__filamentous phage display 1 rec.
           |   |__p3 filamentous phage display
           |   |__p8 filamentous phage display
           |__lambda phage display 15 rec.
           |__t7 phage display

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Is a	display technology
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Synonyms & Secondary IDs
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